Oral
enzyme therapy in the animal model of collagen-II induced arthritis
in mice
Stauder G. Oral
enzyme therapy in the animal model of collagen-II induced arthritis
in mice. Reumatologia 1998, Suppl Vol. XXXVI, Warsaw 1998, Lectures
No. 210, pp 106-107. ISSN 0034-6233. 619 KA (19-09-2)
Mucos Pharma Intl., Geretsried, Germany. 2nd Central European
Congress of Rheumatology, 13-16 May, 1998, Warsaw, Poland
Male DBA/1 mice, 5 weeks old, 20 g bodyweight,
were immunized by subcutaneous injection of collagen II in complete
Freund's adjuvant at the base of the tail.
After 21 days the immunization was repeated with collagen II in
incomplete Freund's adjuvant. Age-matched control mice remained
totally unmanipulated.
From day 28, the immunized mice were treated either with orally
applied Phlogenzym®, or ibuprofen, or were left
untreated.
At various times after, each foot from the animals was checked for
swelling and redness, and documented as score. Additionally,
selected feet from each mouse
were measured in the transversal and saggital planes. After
exsanguination under ether anesthesia serum samples and lymph nodes
were collected. Some mice were investigated by radiography and
histology.
The signs of the arthritis - as clinical score - were reduced
significantly in both treatment groups, as compared to the untreated
mice (table 1). The swelling and redness, however, was in both
treated groups still more present than in the healthy control group
(i.e. non-immunized mice). The clinical score did not
differ between the two treatment groups.
Histologically, the joints were nearly totally obliterated in the
untreated group. Granulation tissue filled the joint space, the
cartilage was destroyed.
Joints in the treated groups were significantly less damaged.