The
effect of enzyme therapy and natrium alendronan on ovariectomized
female rats
Švík K.,
Rovenská E., Stanèíková M., Rovenský J. The effect of enzyme therapy
and natrium alendronan on ovariectomized female rats. The Research
Institute of Rheumatic Diseases, Piešany, SR.
3. Kongres slovenských a èeských osteológov + IOF 2000, Banská
Bystrica, 7.-9. sept., poster str. 48
The goal of this study was to investigate the
impact of the enzyme mixture (bromelain, trypsin and rutin) of the
same composition as Phlogenzym® (PHL) and the trihydrate
natrium alendronan (ALN) in the prophylactic treatment of
postmenopausal osteoporosis in animal model and the possibility to
reduce ALN doses during the combined therapy with PHL.
Adult Sprague Dawley female rats (250 ± 10 g) were bilaterally
ovariectomized (OVX) and sham operated (SHAM). Then they were
divided into 6 groups each containing 8 animals: controls SHAM and
OVX, treated OVX females: Phlogenzym® 90 mg/kg (PHL),
alendronan 500 m g /kg (ALN-500), alendronan 250 m g /kg (ALN-250),
and combination (PHL+ALN-250). PHL was administered per rectum daily
for 9 weeks and ALN per os 5 days a week for the same time. Standard
food was fed according to the daily need of rats. Two parameters
were followed up in the study: bone mineral density (BMD) and bone
mineral content (BMD) of the whole body and femur.
Both BMD and BMC of the whole body and femur in SHAM controls, OVX
alendronan treated were significantly increased in comparison to OVX
and PHL groups. The values of percentage increase of BMD of the
whole body were significantly higher in all treated groups as
compared to the OVX controls. The similar prophylactic effect of
ALN-250, ALN-500 and PHL + ALN- 250 on BMC was noticed.
Our results suggest that the only Phlogenzym® reduces the
decrease of BMD in female rats and has a similar prophylactic effect
as ALN 500 m g/kg if combined with ALN 250 m g/kg.